Addition of ascorbate during cryopreservation stimulates subsequent embryo development
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چکیده
منابع مشابه
melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice
background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...
متن کاملIntegrity rate of pronuclei after cryopreservation of pronuclear-zygotes as a criteria for subsequent embryo development and pregnancy.
BACKGROUND The aim of this study was to examine whether the integrity rate of pronuclei, after cryopreservation of pronuclear zygotes, could be a predictor of future embryo development and implantation. METHODS Two-pronuclei stage zygotes (n = 862) were cryopreserved by aseptic rapid freezing in 15% ethylene glycol + 15% DMSO + 0.2 M sucrose with a 4-step exposure in 12% (v:v), 25, 50 and 100...
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1. Summary 1. Embryonic Development and Viability in Livestock Species 1.1. Techniques for analyzing maternal contributions to embryonic viability 1.2. Physiological basis of embryonic viability in the female genital tract 2. Evaluation of Embryonic Viability 2.
متن کاملEmbryo cryopreservation and development: facts, questions and responsibility.
Four years ago, the results of an experimental study led us to conclude that in the mouse, cryopreservation of preimplantation embryo induced post-natal effects and thus could not be considered as neutral for the long-term development (Dulioust et al., 1995). This raised some concern in the scientific community and in the public about the safety of embryo cryopreservation in human assisted proc...
متن کاملHuman Oocyte And Embryo Cryopreservation
The technology so far applied clinically has been based directly on traditional human embryo cryopreservation protocols, and has produced relatively few offspring. Fortunately to date, no abnormalities have been reported from these pregnancies, regardless of the persistent concerns that freezing and thawing of mature oocytes may disrupt the meiotic spindle and thus increase the potential for an...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2002
ISSN: 1460-2350
DOI: 10.1093/humrep/17.10.2686